BMS503 Techniques in Genetic Engineering (16)
CSU Discipline Area: Biomedical Science (BIMSC)
Duration: One session
Abstract:
This subject will introduce students to bacterial and eukaryotic genetics. This will be followed by in depth study of genetic engineering and related innovative applications in modern medical biotechnology. A short introduction into bioinformatics will be provided as well.
+ Subject Availability Modes and Locations
No offerings have been identified for this subject in 2013.Continuing students should consult the SAL for current offering details prior to contacting their course coordinator: BMS503
Where differences exist between the handbook and the SAL, the SAL should be taken as containing the correct subject offering details.
Assumed Knowledge:
Enrolment restrictions:
Master of Corporate Biotechnology students only
Objectives:
Upon successful completion of this subject, students should:
* Describe basic mechanisms in Eukaryotic and Prokaryotic genetics
* Have a sound understanding in the genetics of extrachromosomal DNA in prokaryotes
* describe and understand a range of techniques in genetic manipulation, the cloning vectors available and the containment procedures required;
* identify strategies for gene isolation, construction of libraries, DNA and peptide synthesis and DNA sequencing;
* Have a full understanding of the complexities associated with engineering the expression of recombinant genes of medical or therapeutic importance, eg, human insulin, epidermal growth factor, human somatotrophin, human dystrophin genes;
* Appreciate the difficulties in cell culture at industrial levels
* describe the diagnostic and medical applications of recombinant DNA technology;
* outline the range of genes implicated in common medical disorders and the variety of therapeutic, diagnostic and preventative methods developed to combat disease;
* begin to appreciate the regulating and patenting of molecular biotechnology products
Syllabus:
The subject will cover the following topics:
* Genomes (viral, prokaryotic, eukaryotic and human); * Genetic analysis of prokaryotes; * Genetics of eukaryotes; * tools of genetic manipulation I: restriction endonucleases, methylases, nucleases, polymerases, ribonucleases, exonucleases, transcriptases, ligases, kinases. Uses of primers, adaptors, linkers; * Tools of Genetic Manipulation II: DNA (Phosphite triester) and Peptide Synthesis, protein engineering, oligonucleotides, peptide pharmaceuticals and automated synthesis. In vitro mutagenesis and elucidation of gene function; * strategies for gene isolation: cloning DNA libraries and genomic DNA libraries, screening for genes of interest; * expression engineering and gearing up of production; * application of biotechnology: human germ-line and somatic gene therapy; transgenic livestock for industry; transgenic animals as pharmaceutical factories; peptide vaccines, peptide pharmaceuticals, vaccinia viruses, antibiotics, recombinant vaccines, food and beverage improvement;. * introduction to regulating and patenting molecular biotechnology
The information contained in the 2013 CSU Handbook was accurate at the date of publication: 24 April 2013. The University reserves the right to vary the information at any time without notice.