BMS304 Fundamentals of DNA Fingerprinting (8)
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This subject gives an overview of the cellular, biochemical, genetic and molecular foundations of human diversity. The underlying principles behind the detection and analysis of phenotypic and genotypic variations will be examined. |
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+ Subject Availability Modes and Location
| Session 2 | | Distance* | Wagga Wagga Campus |
*This subject offering contains a residential school. Please view following information for further details.
Continuing students should consult the SAL for current offering details: BMS304
Where differences exist between the Handbook and the SAL, the SAL should be taken as containing the correct subject offering details.
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Subject information| Duration | Grading System | School: |
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| One session | HD/FL | School of Biomedical Sciences |
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| Incompatible subject(s) |
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| BMS414 |
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Learning OutcomesUpon successful completion of this subject, students should:
- be able to describe the concept of DNA fingerprinting and DNA typing
- be able to define principles behind DNA fingerprinting methodologies currently in use (STR,VNTR,SNPs , PCR and RFLP, Y-chromosome (Y-STR) and mitochondrial analysis
- be able to describe and extract data from DNA databases
- be able to describe the analytical methods behind a number of common genetic tests currently used for forensic human identification purposes
- be able to demonstrate ability to calculate likelihood ratios from typing profiles and understand the influence of population frequencies and other aspects of population genetics on the validity and discriminatory ability of DNA typing methods
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SyllabusThe subject will cover the following topics:
- Identification of biological fluids
- The determination of species origin of biological materials
- The differentiation of biological samples of human origin
- Fundamentals of DNA fingerprinting Technology
- The technologies and methodologies behind the detection and analysis of hypervariable sequences; STR, VNTR, PCR, RFLP and SNPs
- Common PCR-based genetic tests used in forensic practice
- Mitochondrial DNA sequencing identification through maternal lineage
- Y-chromosome STRs - identification through paternal lineage
- Phylogenetic profiling and its application to non-human identification
- Population genetics and statistical considerations
- DNA Technology in its application to medical diagnostics and medical research
- Applying DNA technologies to medicine
- Ethical dilemmas
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Residential SchoolThis subject contains a compulsory 4 day residential school.
Residential school will include the following :
-Students will be divided in groups and each group will be allocated a forensic scenario to solve. Students will use the following laboratory techniques to solve their case:
-Physical examination of evidence and selection of evidence suitable for further analysis.
-Presumptive screening for Blood
-DNA extraction and purification.
-Gel electrophoresis of extracted DNA
-DNA profiling PCR's (Short Tandem Repeats, STRs)
-Analysis of DNA profiling results
-Laboratory Report - At the end of the residential school each student is required to submit a report outlining their group's strategies and experimental results/discussion and a conclusion.
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The information contained in the 2016 CSU Handbook was accurate at the date of publication: 06 September 2016. The University reserves the right to vary the information at any time without notice.
