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BMS415 Genetic Engineering & Bioinformatics (8)

Abstract

An advanced level subject with an intensive practical schedule that familiarises students with techniques used in gene manipulation or genetic engineering and related innovative applications in modern medical biotechnology. This subject will also provide an introduction to bioinformatics.

+ Subject Availability Modes and Location

Continuing students should consult the SAL for current offering details prior to contacting their course coordinator: BMS415
Where differences exist between the handbook and the SAL, the SAL should be taken as containing the correct subject offering details.

Subject information

Duration Grading System School:
One sessionHD/FLSchool of Biomedical Sciences

Assumed Knowledge

BMS241

Enrolment restrictions

Not available to students enrolled in 3 year Bachelor's degree courses.
Related subject(s)
BMS305 BMS415 is a paired subject with BMS305

Learning Outcomes

Upon successful completion of this subject, students should:
* be able to describe the range of techniques in genetic manipulation, the cloning vectors available and the containment procedures required;
* be able to identify strategies for gene isolation, construction of libraries, DNA and peptide synthesis and DNA sequencing;
* abe able to demonstrate understanding of the complexities associated with engineering the expression of recombinant genes of medical or therapeutic importance, eg, human insulin, epidermal growth factor, human somatomammotrophin, human dystrophin genes;
* be able to describe the diagnostic, forensic, and medical applications of recombinant DNA technology.

Syllabus

The subject will cover the following topics:
The syllabus will cover the following topics: * review of basic molecular biology: theory, cloning vectors, mechanism of propagation; * tools of genetic manipulation: restriction endonucleases, methylases, nucleases, polymerases, ribonucleases, exonucleases, transcriptases, ligases, kinases. Uses of primers, adaptors, linkers; * cloning: cleavage of vector and insert, site compatibility, dephosphorylation of ends, ligation protocols and diagnostic techniques; * preparation of transformed cells: transformation and transfection techniques, routine screening procedures; * gene probing: hybridisation methods. Applications in biotechnology; * strategies for gene isolation: cloning DNA libraries and genomic DNA libraries, screening for genes of interest by hybridisation and immuno-detection methods. * Expression engineering: promoters, enhancers, homeo-boxes, fundamental regulatory elements. Secretion of engineered gene products; major factors affecting viability - refractile bodies, post-translational modification. Production, purification and analysis. Perspectives: development of expression vectors for mammalian cells; immunogenicity of engineered proteins - risk assessment in therapy; * DNA (Phosphite triester), protein engineering, oligonucleotides, peptide pharmaceuticals and automated synthesis. In vitro mutagenesis and elucidation of gene function *Genetic therapy: human germ-line and somatic gene therapy; transgenic livestock for industry; transgenic animals as pharmaceutical factories * DNA Sequencing: Sanger dideoxy, M13 and plasmid, troubleshooting, direct genomic sequencing with polymerase chain reaction. Perspectives: The Genome Sequencing Project; automated sequenators; * Scaling up mammalian and bacterial expression cultures: fermentation systems: batch, continuous and fed batch systems, turbidostat and chemostat cultures, downstream processing, inclusion bodies, secreted peptides, animal cell culture technology; * applications in diagnostics, forensics medicine and agriculture: peptide vaccines, peptide pharmaceuticals, vaccinia viruses, antibiotics, recombinant vaccines, food and beverage improvement.

Residential School

This subject contains a compulsory 4 day residential school. Completion of compulsory laboratory experiments.

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The information contained in the 2018 CSU Handbook was accurate at the date of publication: 20 October 2017. The University reserves the right to vary the information at any time without notice.