BMS415 Genetic Engineering & Bioinformatics (8)
An advanced level subject with an intensive practical schedule that familiarises students with techniques used in gene manipulation or genetic engineering and related innovative applications in modern medical biotechnology. This subject will also provide an introduction to bioinformatics.
No offerings have been identified for this subject in 2019.
Continuing students should consult the SAL for current offering details. Where differences exist between the Handbook and the SAL, the SAL should be taken as containing the correct subject offering details.
Subject Information
Grading System
HD/FL
Duration
One session
School
School of Biomedical Sciences
Enrolment restrictions
Not available to students enrolled in 3 year Bachelor's degree courses.
Assumed Knowledge
BMS241
Subject Relationships
BMS305 BMS415 is a paired subject with BMS305
Learning Outcomes
Upon successful completion of this subject, students should:
  • be able to describe the range of techniques in genetic manipulation, the cloning vectors available and the containment procedures required;
  • be able to identify strategies for gene isolation, construction of libraries, DNA and peptide synthesis and DNA sequencing;
  • abe able to demonstrate understanding of the complexities associated with engineering the expression of recombinant genes of medical or therapeutic importance, eg, human insulin, epidermal growth factor, human somatomammotrophin, human dystrophin genes;
  • be able to describe the diagnostic, forensic, and medical applications of recombinant DNA technology.
Syllabus
The subject will cover the following topics:
The syllabus will cover the following topics: * review of basic molecular biology: theory, cloning vectors, mechanism of propagation; * tools of genetic manipulation: restriction endonucleases, methylases, nucleases, polymerases, ribonucleases, exonucleases, transcriptases, ligases, kinases. Uses of primers, adaptors, linkers; * cloning: cleavage of vector and insert, site compatibility, dephosphorylation of ends, ligation protocols and diagnostic techniques; * preparation of transformed cells: transformation and transfection techniques, routine screening procedures; * gene probing: hybridisation methods. Applications in biotechnology; * strategies for gene isolation: cloning DNA libraries and genomic DNA libraries, screening for genes of interest by hybridisation and immuno-detection methods. * Expression engineering: promoters, enhancers, homeo-boxes, fundamental regulatory elements. Secretion of engineered gene products; major factors affecting viability - refractile bodies, post-translational modification. Production, purification and analysis. Perspectives: development of expression vectors for mammalian cells; immunogenicity of engineered proteins - risk assessment in therapy; * DNA (Phosphite triester), protein engineering, oligonucleotides, peptide pharmaceuticals and automated synthesis. In vitro mutagenesis and elucidation of gene function *Genetic therapy: human germ-line and somatic gene therapy; transgenic livestock for industry; transgenic animals as pharmaceutical factories * DNA Sequencing: Sanger dideoxy, M13 and plasmid, troubleshooting, direct genomic sequencing with polymerase chain reaction. Perspectives: The Genome Sequencing Project; automated sequenators; * Scaling up mammalian and bacterial expression cultures: fermentation systems: batch, continuous and fed batch systems, turbidostat and chemostat cultures, downstream processing, inclusion bodies, secreted peptides, animal cell culture technology; * applications in diagnostics, forensics medicine and agriculture: peptide vaccines, peptide pharmaceuticals, vaccinia viruses, antibiotics, recombinant vaccines, food and beverage improvement.
Residential School
This subject contains a 4 day Compulsory Residential School.Completion of compulsory laboratory experiments.
Contact
Current Students

For any enquiries about subject selection or course structure you will need to contact your Course Director. You can find the name and contact details for your Course Director in your offer letter or contact your School office.

Prospective Students

For further information about Charles Sturt University, or this course offering, please contact info.csu on 1800 334 733 (free call within Australia) or enquire online.

The information contained in the 2017 CSU Handbook was accurate at the date of publication: June 2018. The University reserves the right to vary the information at any time without notice.

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