BMS503 Techniques in Genetic Engineering (16)
This subject will introduce students to bacterial and eukaryotic genetics. This will be followed by in depth study of genetic engineering and related innovative applications in modern medical biotechnology. A short introduction into bioinformatics will be provided as well.
No offerings have been identified for this subject in 2019.
Continuing students should consult the SAL for current offering details. Where differences exist between the Handbook and the SAL, the SAL should be taken as containing the correct subject offering details.
Subject Information
Grading System
One session
School of Biomedical Sciences
Enrolment restrictions
Master of Corporate Biotechnology students only
Assumed Knowledge
Subject Relationships
Learning Outcomes
Upon successful completion of this subject, students should:
  • Describe basic mechanisms in Eukaryotic and Prokaryotic genetics
  • Have a sound understanding in the genetics of extrachromosomal DNA in prokaryotes
  • describe and understand a range of techniques in genetic manipulation, the cloning vectors available and the containment procedures required;
  • identify strategies for gene isolation, construction of libraries, DNA and peptide synthesis and DNA sequencing;
  • Have a full understanding of the complexities associated with engineering the expression of recombinant genes of medical or therapeutic importance, eg, human insulin, epidermal growth factor, human somatotrophin, human dystrophin genes;
  • Appreciate the difficulties in cell culture at industrial levels
  • describe the diagnostic and medical applications of recombinant DNA technology;
  • outline the range of genes implicated in common medical disorders and the variety of therapeutic, diagnostic and preventative methods developed to combat disease;
  • begin to appreciate the regulating and patenting of molecular biotechnology products
The subject will cover the following topics:
* Genomes (viral, prokaryotic, eukaryotic and human); * Genetic analysis of prokaryotes; * Genetics of eukaryotes; * tools of genetic manipulation I: restriction endonucleases, methylases, nucleases, polymerases, ribonucleases, exonucleases, transcriptases, ligases, kinases. Uses of primers, adaptors, linkers; * Tools of Genetic Manipulation II: DNA (Phosphite triester) and Peptide Synthesis, protein engineering, oligonucleotides, peptide pharmaceuticals and automated synthesis. In vitro mutagenesis and elucidation of gene function; * strategies for gene isolation: cloning DNA libraries and genomic DNA libraries, screening for genes of interest; * expression engineering and gearing up of production; * application of biotechnology: human germ-line and somatic gene therapy; transgenic livestock for industry; transgenic animals as pharmaceutical factories; peptide vaccines, peptide pharmaceuticals, vaccinia viruses, antibiotics, recombinant vaccines, food and beverage improvement;. * introduction to regulating and patenting molecular biotechnology
Current Students

For any enquiries about subject selection or course structure you will need to contact your Course Director. You can find the name and contact details for your Course Director in your offer letter or contact your School office.

Prospective Students

For further information about Charles Sturt University, or this course offering, please contact info.csu on 1800 334 733 (free call within Australia) or enquire online.

The information contained in the 2017 CSU Handbook was accurate at the date of publication: June 2018. The University reserves the right to vary the information at any time without notice.