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BMS503 Techniques in Genetic Engineering (16)

Abstract

This subject will introduce students to bacterial and eukaryotic genetics. This will be followed by in depth study of genetic engineering and related innovative applications in modern medical biotechnology. A short introduction into bioinformatics will be provided as well.

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Continuing students should consult the SAL for current offering details prior to contacting their course coordinator: BMS503
Where differences exist between the handbook and the SAL, the SAL should be taken as containing the correct subject offering details.

Subject information

Duration Grading System School:
One sessionHD/FLSchool of Biomedical Sciences

Assumed Knowledge

BMS502

Enrolment restrictions

Master of Corporate Biotechnology students only

Learning Outcomes

Upon successful completion of this subject, students should:
* Describe basic mechanisms in Eukaryotic and Prokaryotic genetics
* Have a sound understanding in the genetics of extrachromosomal DNA in prokaryotes
* describe and understand a range of techniques in genetic manipulation, the cloning vectors available and the containment procedures required;
* identify strategies for gene isolation, construction of libraries, DNA and peptide synthesis and DNA sequencing;
* Have a full understanding of the complexities associated with engineering the expression of recombinant genes of medical or therapeutic importance, eg, human insulin, epidermal growth factor, human somatotrophin, human dystrophin genes;
* Appreciate the difficulties in cell culture at industrial levels
* describe the diagnostic and medical applications of recombinant DNA technology;
* outline the range of genes implicated in common medical disorders and the variety of therapeutic, diagnostic and preventative methods developed to combat disease;
* begin to appreciate the regulating and patenting of molecular biotechnology products

Syllabus

The subject will cover the following topics:
* Genomes (viral, prokaryotic, eukaryotic and human); * Genetic analysis of prokaryotes; * Genetics of eukaryotes; * tools of genetic manipulation I: restriction endonucleases, methylases, nucleases, polymerases, ribonucleases, exonucleases, transcriptases, ligases, kinases. Uses of primers, adaptors, linkers; * Tools of Genetic Manipulation II: DNA (Phosphite triester) and Peptide Synthesis, protein engineering, oligonucleotides, peptide pharmaceuticals and automated synthesis. In vitro mutagenesis and elucidation of gene function; * strategies for gene isolation: cloning DNA libraries and genomic DNA libraries, screening for genes of interest; * expression engineering and gearing up of production; * application of biotechnology: human germ-line and somatic gene therapy; transgenic livestock for industry; transgenic animals as pharmaceutical factories; peptide vaccines, peptide pharmaceuticals, vaccinia viruses, antibiotics, recombinant vaccines, food and beverage improvement;. * introduction to regulating and patenting molecular biotechnology

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The information contained in the 2018 CSU Handbook was accurate at the date of publication: 18 October 2017. The University reserves the right to vary the information at any time without notice.