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Brett McVittie
Masters Student
School of Agricultural and Wine Sciences
Location Charles Sturt University, Wagga Wagga
Phone 02 6938 1660
Fax 02 6938 1809
Email bmcvittie@csu.edu.au
Project
Identification of candidate genes involved in Manganese Tolerance in Canola (Brassica napus L).
Summary
Canola is the third largest crop in Australia after wheat and barley, and the most significant oilseed crop. It is grown predominantly for the export market with significant potential for increased exports. However, it is widely considered to be sensitive to soil acidity and yields poorly at pH less than 5.0. Al and Mn toxicities are recognised as the primary factors limiting canola production on acidic soils. Nevertheless, significant genetic variation for Mn tolerance exists in the canola germplasm.
Researchers at I&I NSW and the EH Graham Centre have already developed non-destructive screening techniques for evaluation of canola germplasm for Mn tolerance. These researchers have identified at least 16 genotypes of canola having tolerance to high Mn and demonstrated that this trait appeared to be controlled by a single gene in an F 2 population. However the location of the gene encoding Mn tolerance, on the canola chromosome is unknown yet.
The objectives of the proposed research are:
- To locate gene(s) involved in Mn tolerance in an F 2 population of canola using molecular markers
- Determine allele diversity at Mn tolerance locus in canola germplasm
- Identify Mn homologues using Arabidopsis synteny and develop functional gene marker associated with Mn tolerance.
Supervisors
Dr Harsh Raman, Dr John Harper, Dr Sergio Moroni, Neil Wratten