This subject contains a 4 day Compulsory Residential School.Completion of compulsory laboratory experiments.
An advanced level subject with an intensive practical schedule that familiarises students with techniques used in gene manipulation or genetic engineering and related innovative applications in modern medical biotechnology. This subject will also provide an introduction to bioinformatics.
No offerings have been identified for this subject in 2019.
HD/FL
One session
School of Biomedical Sciences
Not available to students enrolled in 3 year Bachelor's degree courses.
BMS305 BMS415 is a paired subject with BMS305
The syllabus will cover the following topics: * review of basic molecular biology: theory, cloning vectors, mechanism of propagation; * tools of genetic manipulation: restriction endonucleases, methylases, nucleases, polymerases, ribonucleases, exonucleases, transcriptases, ligases, kinases. Uses of primers, adaptors, linkers; * cloning: cleavage of vector and insert, site compatibility, dephosphorylation of ends, ligation protocols and diagnostic techniques; * preparation of transformed cells: transformation and transfection techniques, routine screening procedures; * gene probing: hybridisation methods. Applications in biotechnology; * strategies for gene isolation: cloning DNA libraries and genomic DNA libraries, screening for genes of interest by hybridisation and immuno-detection methods. * Expression engineering: promoters, enhancers, homeo-boxes, fundamental regulatory elements. Secretion of engineered gene products; major factors affecting viability - refractile bodies, post-translational modification. Production, purification and analysis. Perspectives: development of expression vectors for mammalian cells; immunogenicity of engineered proteins - risk assessment in therapy; * DNA (Phosphite triester), protein engineering, oligonucleotides, peptide pharmaceuticals and automated synthesis. In vitro mutagenesis and elucidation of gene function *Genetic therapy: human germ-line and somatic gene therapy; transgenic livestock for industry; transgenic animals as pharmaceutical factories * DNA Sequencing: Sanger dideoxy, M13 and plasmid, troubleshooting, direct genomic sequencing with polymerase chain reaction. Perspectives: The Genome Sequencing Project; automated sequenators; * Scaling up mammalian and bacterial expression cultures: fermentation systems: batch, continuous and fed batch systems, turbidostat and chemostat cultures, downstream processing, inclusion bodies, secreted peptides, animal cell culture technology; * applications in diagnostics, forensics medicine and agriculture: peptide vaccines, peptide pharmaceuticals, vaccinia viruses, antibiotics, recombinant vaccines, food and beverage improvement.
This subject contains a 4 day Compulsory Residential School.Completion of compulsory laboratory experiments.
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The information contained in the CSU Handbook was accurate at the date of publication: May 2019. The University reserves the right to vary the information at any time without notice.