BMS304 Fundamentals of DNA Fingerprinting (8)

This subject gives an overview of the cellular, biochemical, genetic and molecular foundations of human diversity. The underlying principles behind the detection and analysis of phenotypic and genotypic variations will be examined.

Subject availability
* Offering has a residential school. Please view following information for further details.
Session 2 (60)
Online *
Wagga Wagga Campus
Continuing students should consult the SAL for current offering details. Where differences exist between the Handbook and the SAL, the SAL should be taken as containing the correct subject offering details.
Subject Information
Grading System
HD/FL
Duration
One session
School
School of Biomedical Sciences
Assumed Knowledge

BMS241

Subject Relationships
Incompatable Subjects
BMS414
Learning Outcomes
Upon successful completion of this subject, students should:
  • be able to describe the concept of DNA fingerprinting and DNA typing
  • be able to define principles behind DNA fingerprinting methodologies currently in use (STR,VNTR,SNPs , PCR and RFLP, Y-chromosome (Y-STR) and mitochondrial analysis
  • be able to describe and extract data from DNA databases
  • be able to describe the analytical methods behind a number of common genetic tests currently used for forensic human identification purposes
  • be able to demonstrate ability to calculate likelihood ratios from typing profiles and understand the influence of population frequencies and other aspects of population genetics on the validity and discriminatory ability of DNA typing methods
Syllabus
The subject will cover the following topics:
  • Identification of biological fluids
  • The determination of species origin of biological materials
  • The differentiation of biological samples of human origin
  • Fundamentals of DNA fingerprinting Technology
  • The technologies and methodologies behind the detection and analysis of hypervariable sequences; STR, VNTR, PCR, RFLP and SNPs
  • Common PCR-based genetic tests used in forensic practice
  • Mitochondrial DNA sequencing identification through maternal lineage
  • Y-chromosome STRs - identification through paternal lineage
  • Phylogenetic profiling and its application to non-human identification
  • Population genetics and statistical considerations
  • DNA Technology in its application to medical diagnostics and medical research
  • Applying DNA technologies to medicine
  • Ethical dilemmas
Residential School
This subject contains a 4 day Compulsory Residential School.

Residential school will include the following :

-Students will be divided in groups and each group will be allocated a forensic scenario to solve. Students will use the following laboratory techniques  to solve their case: 

-Physical examination of evidence and selection of evidence suitable for further analysis. 

-Presumptive screening for Blood
-DNA extraction and purification.
-Gel electrophoresis of extracted DNA
-DNA profiling PCR's (Short Tandem Repeats, STRs)
-Analysis of DNA profiling results

-Laboratory Report - At the  end of the residential  school each student is required to submit a report outlining their group's strategies and experimental results/discussion and a conclusion.

 

Contact
Current Students

For any enquiries about subject selection or course structure you will need to contact your Course Director. You can find the name and contact details for your Course Director in your offer letter or contact your School office.

Prospective Students

For further information about Charles Sturt University, or this course offering, please contact info.csu on 1800 334 733 (free call within Australia) or enquire online.

The information contained in the 2017 CSU Handbook was accurate at the date of publication: June 2018. The University reserves the right to vary the information at any time without notice.

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